Anna of St Augustine was born in Valladolid on the 11^th of December, 1555.

In 1577 she was accepted at the Carmelite monastery of Malagon (Spain) where in 1578 she made her solemn profession.  Favoured by special mystical experiences, in 1579 she was examined by St. Teresa of Avila who found her revelations authentic and entrusted her with the foundation of the monastery of Villanueva de la Jara of which she also became prioress. Later she was again transferred to the monastery of Valera de Abajo. In 1616 she returned as prioress to the monastery of Villanueva where she spent her last years. 

She died in 1624.

The decree on her heroic virtue was promulgated on 15^th September, 1776.